Molecular Analysis of Agaricus bisporus Double-stranded RNA
نویسندگان
چکیده
منابع مشابه
Double-stranded RNAs and proteins associated with the 34 nm virus particles of the cultivated mushroom Agaricus bisporus.
Agaricus bisporus fruit bodies affected by La France disease contain a specific set of nine dsRNA molecules. A method was developed to isolate intact virus particles containing these dsRNAs. Using precautions to limit proteolysis, virus particles 34 nm in diameter were banded in a Nycodenz gradient together with the nine disease-associated dsRNAs and three proteins of M(r) 120K, 115K and 90K. T...
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Agrobacterium tumefaciens is known to transfer parts of its tumor-inducing plasmid to filamentous fungi including Agaricus bisporus. We have used this transformation system to transform germinating basidiospores of A. bisporus. Analysis of hygromycin B-resistant clones showed that the T-DNA integrated at random sites and in multiple copies into the host genome. Furthermore we have been able to ...
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The molecular weight of the double-stranded RNA (Pl dsRNA) always associated with the virus-like particles that occur in the yeast Saccharomyces cerevisiae was determined to be 3.5 + 0.1 X lo6 by equilibrium sedimentation. Methylmercuric-agarose gel electrophoresis yielded a value of 1.67 + 0.11 x lo6 for the single-stranded form. The sedimentation coefficient, 0 SZO+,, was determined by bounda...
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The molecular weight of the double-stranded RNA (Pl dsRNA) always associated with the virus-like particles that occur in the yeast Saccharomyces cerevisiae was determined to be 3.5 + 0.1 X lo6 by equilibrium sedimentation. Methylmercuric-agarose gel electrophoresis yielded a value of 1.67 + 0.11 x lo6 for the single-stranded form. The sedimentation coefficient, 0 SZO+,, was determined by bounda...
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Partially purified virus preparations from sporophores of Agaricus bisporus affected with LaFrance disease had up to a 15-fold-higher RNA-dependent RNA polymerase activity than did comparable preparations from healthy sporophores. Enzyme activity was dependent upon the presence of Mg2+ and the four nucleoside triphosphates and was insensitive to actinomycin D, a-amanitin, and rifampin. The 3H-l...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1989
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-70-6-1613